Spec 64

Parameters - Datasheet

Rotating motorized (single) grating

Operation in the wavelength range from 400 nm to 1100 nm (with appropriate gratings)

Resolution of 5 cm^-1 with a spectral window of 2100 cm^-1at 532 nm

High-speed imaging with an acquisition time down to 10 ms per spectrum

Calibration lamp for spectrometer calibration or high precision measurements (see Software / Accessories)

Software package for acquisition and data analysis of single spectra or Raman mappings Synchronization to Anfatec AFM software and MoleculaVista VistaScan (see VistaScope)

A detailed description of applications and examples can be found on the Spec64 performance page.

Go to: Spectrometer Performance
Specifications
Software and Accessories

The fiber coupled high speed Raman spectrometer is optimized for maximum light throughput and high speed spectrum acquisition. Connected to an AFM or a scanning stage, high speed Raman imaging is possible due to an integration time down to 10 ms per spectrum. The spectrometer can be operated with a separate software as stand-alone device or synchronized via the Anfatec AFM software.

Spectrometer Performance

Raman Spectrum of Silicon Wafer

A silicon wafer can be utilized as benchmark sample for spectrometer performance. A signal to noise ratio of more than 300 could be obtained for the main silicon phonon at 520 cm^-1.

Raman spectrum of silicon measured with the Spec64 and a total exposure time of 500 ms. A 100x oil objective (1.25 NA) was utilied for light collection. The laser power at the sample was less than 4 mW at 532 nm.

Raman Imaging on Polystyrene Spheres

The Raman map of self-assebled polystyrene spheres with a diameter of 850 nm illustrates the spatial resolution of confocal Raman imaging.

The left image shows a intensity map of the Raman spectrum of polystyrene spheres. The image size is 30 µm x 30 µm with a pixel resolution of 128 x 128. A microscope image of the measured area is displayed on the right.

Raman Mapping of Single CNTs with a Gold Tip Approached (TERS Configuration)

A highly deluted dispersion of (6,5) semiconducting CNTs was dropcoated on a glass slide. A 532 nm laser (optical power of 1 mW to 2 mW) was focussed on the apex of a full metal gold tip via the bottom objective. The distance of the oscillating tip and the sample is kept constant via the atomic force feedback (AFM) loop of the VistaScope. Laser focus and tip remains fixed while the sample is scanned.

The 10 µm x 10 µm image shows the intensity of the G peak of CNTs. The spectra displayed on the right show a high signal to noise ratio with an acquisition time of only 100 ms.

Specifications of the High-Speed Raman Spectrometer Spec64

Optical Characteristics

Spectrometer layout	Single grating Czerny-Turner spectrometer
Focal length	200 mm
Numerical aperture	max. 0.12
Grating size	50 mm x 50 mm
Wavelength range	400 nm to 900 nm

Electrical and Mechanical Specifications

Computer interface	USB 2.0
Power supply	12 V DC (provided)
Dimensions	347 mm (L) x 232.5 mm (W) x 155 mm (H)
	Double housing as ambient light protection

Spectral Resolution

with 1800 l/mm grating and 50 µm optical fiber

Center wavelength	532 nm	633 nm	785 nm
Spectral dispersion [nm/mm]	2	1.8	1.4
Technical resolution [cm^-1/pixel]	1	0.64	0.3
Spectrometer resolution [cm^-1]	5	3.5	2
Spectral coverage of full CCD [cm^-1]	2100	1300	650
Spectral coverage of full CCD [nm]	60	52	40

Other gratings are available on request.

Positioning Accuracy

Grating positioning resolution	0.00006°
Wavelength accuracy over full wavelength range	± 0.2 nm
Wavelength repeatability	± 0.01 nm

Characteristics of the CCD

Sensor image size	~ 27 mm x ~ 0.5 mm
Active pixels (horizontal)	2048
Hermetic sealing	Quartz glass window
Time per spectrum	min. 10 ms
CCD node sensitivity	< 8 µV/e^-
CCD dark current at 25 °C	typ. < 80 e^-/pixel/s
Operation Temperature	11 °C ± 0.5 °C
Dynamic range	1:50000
Spectral response range	200 nm to 1100 nm

The image shows the typical quantum efficiency of the CCD without quartz window.

Software and Accessories

Noble Gas Calibration Lamp

Description

The Anfatec Noble Gas Calibration Lamp is ideally suited to calibrate fiber coupled spectrometers. It provides spectrally dense gas emission lines over a wide range of the visible and near infrared spectrum. A list with literature values of the precise spectral position of the emission lines is included. If the lamp slightly illuminates the measured sample, high precision measurements can be performed due to the exactly known emission lines appearing in the measured spectrum.

Image of the calibration lamp with fiber connector at the front and side ports for versatile accessibility.

Summary of the Key Features of the Calibration Lamp

Working principle	Neon and Argon gas emission lines
Wavelength range	~ 590 nm to 900 nm
Fiber connector type	FC/PC
Power supply	12 V DC (provided)

The image shows a typical spectrum of the calibration lamp taken with the Spec64.

Spectrum Acquisition ⇒ CCD.exe

CCD.exe provides the full control over all spectrometer functions including:

Changing CCD center wavelength by rotation of the grating
Initialization and calibration of the spectrometer
Changing exposure time and number of accumulations
Save spectrum or auto-save
Subtraction of a previously measured background spectrum

Initialization procedure of the Spec64 with CCD.exe by adjusting the position of the 0^th order peak.

Spectrum Evaluation ⇒ CCDPresent.exe

CCDPresent.exe is a powerful tool for data evaluation of single spectra or imaging data files.

Single spectrum evaluation:

Polynomial fitting and subtraction of a background
Fitting of peaks
Manual or automatic assignment of fitted peak positions to calibration lamp emission lines
Calculation of deviation of measured peak positions and gas emission line literature values

Automatic fitting of a spectrum of the Anfatec calibration lamp with the Spec64 spectrometer. The graph on the left side shows the deviation of the measured peak positions and gas emission line literature values over the full CDD in units of picometer (the m means millinanometer).

Evaluation of Raman imaging data files:

Plotting an intensity map of a specific spectral range in the spectrum
Showing a specific spectrum selected in the intensity map
Calculating an average spectrum of a selected area
Plotting all spectra of a selected area of the intensity map
Calculating the deviation of measured peak positions and gas emission line literature values

Evaluation of Raman imaging of a pharmaceutical tablet measured with 100 ms exposure time per spectrum with the Spec64 attaced to the VistaScope AFM. The pixel resolution is 32 x 32 pixel in a scan range of 30 µm. The average of a selected area is displayed on the left side. The red marks define a spectral region. The intensity of this specific region is displayed on the right side as intensity plot.